周燕

生物化工专业：博士生导师/硕士生导师

生物工程专业：博士生导师/硕士生导师

生物与医药专业（生物工程领域）：博士生导师/硕士生导师

个人介绍

教授，博士生导师。

1988年获华东化工学院（现3044am永利集团官网）生物化学专业理学学士学位；1991年获3044am永利集团官网生物化工专业工学硕士学位；毕业后留校任教，期间曾在香港城市大学生物及化学系开展研究工作。现任职3044am永利集团官网生物反应器工程国家重点实验室动物细胞与组织工程研究团队，从事动物细胞培养技术研究及其在干细胞/成体细胞体外大规模培养和人体组织体外构建中的应用。承担并完成国家级和省部级科研项目二十余项，在国内外核心刊物发表论文一百二十余篇，申请、公开或授权国家发明专利十多项，获上海市科技进步奖二等奖。

研究方向：

1.体外培养环境调控干细胞扩增和分化的机制

2.干细胞体外规模化扩增和定向分化诱导技术

3.工程化人体组织体外构建新技术及生物反应器研制

4.动物细胞体外培养的无血清培养基设计和开发

5.动物细胞培养过程优化及抗体、病毒疫苗高效生产技术

主持或参加的科研项目或基金：

1.国家重大研发计划：生物力学调控细胞生物学行为及微组织构建

2.国家“863”计划：（1）组织工程种子细胞体外大量扩增技术研究；（2）临床级干细胞产业化技术研发；（3）人体皮肤组织工程研究

3.国家重大新药创制：哺乳动物细胞大规模培养及药物制备关键技术

4.国家支撑计划：动物细胞大规模无血清培养及表达产品制备技术

5.国家自然科学基金：（1）微组织体外构建技术及其过程分析；（2）基于微构重塑技术构建大型预血管化工程组织

6.上海市重大科研攻关：骨髓间充质干细胞体外扩增及成骨诱导的应用研究

7.上海市基础研究重点：（1）hiHep体外规模化扩增；（2）功能性聚酯基组织工程材料制备及其对组织再生调控的研究

8.上海市科技兴农重点攻关：（1）微载体技术用于兽用细胞疫苗产业化研究；（2）猪流行性腹泻、猪传染性胃肠炎和轮状病毒的诊断及三联苗研制

教学：

1.生物技术专业本科生：《细胞工程》

2.3044am永利集团研究生：《细胞培养工程》

近5年代表性论文（*通讯作者）：

1.   Ziyang Jin, Yishu Zhai, Yi Zhou, Pan Guo, Miaomiao Chai, Wensong Tan, Yan Zhou*, Lian Cen*. Regulation of mesenchymal stem cell osteogenic potential via microfluidic manipulation of microcarrier surface curvature. Chemical Engineering Journal, 2022,https://doi.org/10.1016/j.cej.2022.13773.

2.    Ce Gu, Wenjing Du, Miaomiao Chai, Ziyang Jin, Yi Zhou, Pan Guo, Yan Zhou*, Wen-Song Tan. Human umbilical cord-derived mesenchymal stem cells affect urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL-6 in a serum-free co-culture system. Biotechnology Journal. 2022, 17:2100096.

3.     Wenxia Ren, Miaomiao Chai, Mingli Jiang, Yan Zhou*, Wen-Song Tan. High glucose mediates apoptosis and osteogenesis of MSCs via downregulation of AKT-Sirt1-TWIST. Molecular Biology Reports. 2022, 49:2723-2733.

4.    Miaomiao Chai, Mingli Jiang, Ce Gu, Qiaohui Lu, Yi Zhou, Ziyang Jin, Yan Zhou*, Wen-song Tan. Osteogenically differentiated mesenchymal stem cells promote the apoptosis of human umbilical vein endothelial cells in vitro. Biotechnology and Applied Biochemistry. 2022, doi: 10.1002/bab.2274.

5.   Wenjing Du, Ce Gu, Guo Pan, Yan Zhou*, Wen-Song Tan. The adverse effects of hypoxia on hiHep functions via HIF-1 alpha/PGC-1 alpha axis are alleviated by PFDC emulsion. Biochemical Engineering Journal. 2021, 176:108152.

6.   Mingli Jiang, Qihua Shen, Yi Zhou, Wenxia Ren, Miaomiao Chai, Yan Zhou*, Wen-Song Tan. Fluid shear stress and endothelial cells synergistically promote osteogenesis of mesenchymal stem cells via integrin β1-FAK-ERK1/2 pathway. Turkish Journal of Biology. 2021, 45: 683-694

7.   Jiazhu Xu, Qi Fang, Yanli Liu, Yan Zhou*, Zhaoyang Ye*, Wen-Song Tan. In situ ornamenting poly(ε-caprolactone) electrospun fibers with different fiber diameters using chondrocyte-derived extracellular matrix for chondrogenesis of mesenchymal stem cells. Colloids and Surfaces B-Biointerfaces. 2021, 197: 111374.

8.    Chao Wei#, Bingyu Liang#, Yongsheng Li#, Bingkun Yan, Jiaqian Hou, Yan Zhou*, Yingbin Liu*, Meidong Lang*. A drug-free therapeutic system for cancer therapy by diselenide-based polymers themselves. Advanced Healthcare Materials. 2021, 10(2): e2001471.

9.    Miaomiao Chai, Ce Gu, Qihua Shen, Jiaxing Liu, Yi Zhou, Ziyang Jin, Wanli Xiong, Yan Zhou*, Wensong Tan. Hypoxia alleviates dexamethasone-induced inhibition on angiogenesis in co-culture of HUVECs and rBMSCs via HIF-1α. Stem Cell Research & Therapy. 2020, 11(1): 343.

10.  Wei Liu, Mi Zhang, Miaomiao Zhou, Ce Gu, Zhaoyang Ye, Yan Xiao*, Yan Zhou*, Meidong Lang, Wen-Song Tan. Fabrication and evaluation of modified poly(ethylene terephthalate) microfibrous scaffolds for hepatocyte growth and functionality maintenance. Materials Science & Engineering C-Materials for Biological Applications. 2020, 109: 110523.

11.   Wei Liu, Mi Zhang, Yan Xiao, Zhaoyang Ye, Yan Zhou*, Meidong Lang, Wen-Song Tan. Fabrication and in vitro evaluation of a packed-bed bioreactor based on galactosylated poly(ethylene terephthalate) microfibrous scaffolds. Biochemical Engineering Journal. 2020, 160: 107565.

12.   Ce Gu, Miaomiao Chai, Jiaxing Liu, Hui Wang, Wenjing Du, Yan Zhou*, Wen-Song Tan. Expansion of transdifferentiated hepatocyte in a serum-free microcarrier culture system. Digestive Diseases and Sciences. 2020, 65(7): 2009-2023.

13.   Jiaxing Liu, Hui Wang, Wenxia Ren, Yan Zhou*, Wen-Song Tan. β-mercaptoethanol promotes osteogenesis of human mesenchymal stem cells via sirt1-ERK pathway. Cytotechnology. 2020, 72(5): 695-706.

14.   Qihua Shen, Xiaoting Fan, Mingli Jiang, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture. Archives of Biochemistry and Biophysics. 2019, 675: 108108.

15.   Huimin He, Zhaoyang Ye*, Yan Zhou*, Wensong Tan. Dynamic formation of cellular aggregates of chondrocytes and mesenchymal stem cells in spinner flask. Cell Proliferation. 2019, 2019: e12587.

16.   Ce Gu, Panpan Li, Wei Liu, Yan Zhou*, Wen-Song Tan. The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium. Journal of Cellular and Molecular Medicine. 2019, 23(6): 4165-4178.

17.   Jie Sun, Yan Zhou*, Zhaoyang Ye*, Wen-Song Tan. Transforming growth factor-β1 stimulates mesenchymal stem cell proliferation by altering cell cycle through the FAK-Akt-mTOR pathway. Connective Tissue Research. 2019, 60(4): 406-417.

18.   Anmin Zhou, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan. Bioactive Poly(ε-caprolactone) microspheres with tunable open pores as microcarriers for tissue regeneration. Journal of Biomaterials Applications. 2019, 33(9): 1242-1251.

19.   Wei Liu, Dan Hu, Ce Gu, Yan Zhou*, Wen-Song Tan. Fabrication and in vitro evaluation of a packed-bed bioreactor based on an optimum two-stage culture strategy. Journal of Bioscience and Bioengineering. 2019, 127(4): 506-514.

20.    Pan-Pan Li, Ce Gu, Bing-Yu Liang, Lin Wang, Yan Zhou*, Wen-Song Tan. A serum-free medium suitable for maintaining cell morphology and liver-specific function in induced human hepatocytes. Cytotechnology. 2019, 71(1): 329-344.

21.   Yan Xiao*, Miaomiao Zhou, Mi Zhang, Wei Liu, Yan Zhou*, Meidong Lang*. Hepatocyte culture on 3D porous scaffolds of PCL/PMCL. Colloids and Surfaces B-Biointerfaces. 2019, 173: 185-193.

22.   Mengjuan Liu, Jiaqi Wang, Hongping Tang, Li Fan, Liang Zhao, Hai-Bin Wang, Yan Zhou*, Wen-Song Tan. Cell culture medium supplemented with taurine decreases basic charge variant levels of a monoclonal antibody. Biotechnology Letters. 2018, 40(11-12): 1487-1493.

23.   Xiaoting Fan, Yi Teng, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. The effect of gap junction-mediated transfer of miR-200b on osteogenesis and angiogenesis in a co-culture of MSCs and HUVECs. Journal of Cell Science. 2018, 131(13): jcs216135. DOI: 10.1242/ jcs.216135.

24.   Xiaoting Fan, Lei Li, Zhaoyang Ye*, Yan Zhou*, Wensong Tan. Regulation of osteogenesis of human amniotic mesenchymal stem cells by sodium butyrate. Cell Biology International. 2018, 42(4): 457-469.

25.   Songjie Zhang, Min Zhou, Zhaoyang Ye *, Yan Zhou *, Wensong Tan. Fabrication of viable and functional pre-vascularized modular bone tissues by coculturing MSCs and HUVECs on microcarriers in spinner flasks. Biotechnology Journal. 2017, 12(8): 1700008.

专利：

1.     周燕, 卢巧慧, 柴苗苗, 张博韬, 谭文松. 悬浮培养用的搅拌桨及转瓶生物反应器。

2.     周燕，王照闯，郭盼，肖艳，卢巧慧，郎美东，谭文松。一种软骨培养物周期性循环压缩刺激力学装置。

3.     周燕，张咪，刘伟，谭文松一种用于肝细胞三维培养的生物支架及其制备方法和应用

4.     周燕，梁冰玉，杨晨曦，李盼盼，叶朝阳，谭文松适于肝样细胞体外培养的替代血清组合物及其使用方法

5.     周燕，吴伟，谭文松，用于骨髓间充质干细胞体外培养和扩增的无血清培养基

6.     谭文松，赵亮，周燕，黄锭适于MDCK细胞大规模贴壁培养和单细胞悬浮培养的无血清培养基

7.     谭文松，赵亮，范里，刘旭平，周燕，孙亚婷适于动物细胞高密度培养和产物表达的营养物添加剂

8.      牛红星，赵亮，范里，周燕，谭文松，适用于NS0细胞大规模培养及抗体生产的无蛋白培养基

联系方式：

电话：021-64251570

传真：021-64252250

邮箱：zhouyan@ecust.edu.cn

地址：上海市梅陇路130号3044am永利集团官网实验十八楼1013室

信箱：3044am永利集团官网309信箱，邮编200237