李春秀

生物工程专业：博士生导师/硕士生导师

生物学专业：博士生导师/硕士生导师

生物与医药专业（生物工程领域）：博士生导师/硕士生导师

      2005年毕业于中国林科院，获得林木分子生物学专业博士学位。2005年-2008年在中国林科院林木分子遗传重点实验室进行博士后研究。2012年赴日本短期学习嗜热微生物的培养。现于3044am永利集团官网应用生物学系任职副教授，主讲仿生学、生态学、生物化学等课程；同时也是生物催化与合成生物技术研究室的主要成员之一，从事新酶的挖掘改造与催化机制研究以及天然产物的生物合成。作为课题组长主持国家自然科学基金项目2项：“胆酸类药物合成用12α-/7β-羟基甾体脱氢酶的创制改造及构效关系”和“长链烷基糖苷酶基因的克隆表达及催化性能研究”，863项目子课题“羰基还原酶的分子改造”，科技支撑项目“茶油天然脂肪酸的糖基化及其产品性能研究”，上海市教委产业化支撑项目“不对称还原酶法生产降血脂药物中间体”以及企业合作项目两项。近年来累计发表SCI论文78篇，被引用1400余次。申请发明专利21项，授权15项。

研究方向：

1）新酶的挖掘改造与催化机制研究

2）天然产物的生物合成

代表性论文（*通讯作者）：

[1]  You ZN, Zhou K, Han Y, Yang BY, Chen Q, Pan J, Qian XL, Li CX*, Xu JH* (2021). Design of a self-sufficient hydride-shuttling cascade for concurrent bioproduction of 7,12-dioxolithocholate and L-tert-leucine. Green Chem., 2021,23, 4125–4133.

[2]  Zhan JR, Shou C, Zheng YC, Chen Q, Pan J, Li CX*, Xu JH* (2021). Discovery and engineering of bacterial (-)-isopiperitenol dehydrogenases to enhance (-)-menthol precursor biosynthesis. Adv. Synth. Catal., doi.org/10.1002/adsc.202100368.

[3]  Zhang GX,^# You ZN,^# Yu JM, Liu YY, Pan J, Xu JH*, Li CX*(2021). Discovery and engineering of a novel Baeyer-Villiger monooxygenase with high normal regioselectivity. ChemBioChem2021, 22, 1190–1195.

[4]  You ZN,^# Qi Chen,^# Shi SC, Zheng MM, Pan J, Qian XL, Li CX*, and Xu JH* (2019). Switching cofactor dependence of 7β-hydroxysteroid dehydrogenase for cost-effective production of ursodeoxycholic acid. ACS Catal., 2019, 9, 466–473.

[5]  Shi SC,^# You ZN,^# Zhou K, Chen Q, Pan J, Qian XL, Xu JH*, Li CX* (2019). Efficient synthesis of 12-oxochenodeoxycholic acid using a 12α-hydroxysteroid dehydrogenase from Rhodococcus ruber. Adv. Synth. Catal. 2019, 361, 4661–4668.

[6]  Zheng MM, Chen FF, Li H, Li CX*, and Xu JH* (2018). Continuous production of ursodeoxycholic acid by using two cascade reactors with co-immobilized enzymes. ChemBioChem, 2018, 19, 347–353.

[7] Zheng MM, Chen KC, Wang RF, Li H, Li CX*, and Xu JH* (2017). Engineering 7β-hydroxysteroid dehydrogenase for enhanced ursodeoxycholic acid production by multiobjective directed evolution. J. Agric. Food Chem., 65: 1178−1185.

[8]  Chen KC, Zheng MM, Pan J, LiCX*, Xu JH* (2017). Protein engineering and homologous expression of Serratia marcescens Lipase for efficient synthesis of a pharmaceutically relevant chiral epoxyester. Appl Biochem Biotechnol., 183:543–554

[9]  Jiang Y, Shang YP, Li H, Zhang C, Pan J, Bai YP, Li CX* and Xu JH* (2017). Enhancing transglutaminase production of Streptomyces mobaraensis by iterative mutagenesis breeding with atmospheric and room-temperature plasma (ARTP). Bioresour. Bioprocess. 4:37

[10] Liu XY, Chen FF, Li CX^∗, Xiao-Jing Luo, Qi Chen, Yun-Peng Bai, Jian-He Xu (2016).Improved efficiency of a novel methyl parathion hydrolase using consensus approach. Enzyme Microb. Tech., 93-94,11–17

[11] Yi Gong, Guo-Chao Xu, Qi Chen, Jin-Gang Yin, Chun-Xiu Li* and Jian -He Xu* (2016). Iterative Multitarget Evolution Dramatically Enhances the Enantioselectivity and Catalytic Efficiency of Bacillus subtilis Esterase towards Bulky Benzoate Ester of dl-Menthol. Catal. Sci. Technol., 6, 2370–2376

[12] Li CX, Jiang XC, Qiu YJ, Xu JH* (2015). Identification of a new thermo- and alkaline-tolerant α-carbonic anhydrase from Lactobacillus delbrueckii: Performance in CO2 biomineralization into vaterite. Bioresources and Bioprocessing, 2: 44

[13] Du FL, Yu HL, Xu JH, Li CX*(2014). Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli Bioresources and Bioprocessing, 1: 10.

[14] Gong Y, Xu GC, Li CX*, Zheng GW, Xu JH* (2014). A thermostable variant of Bacillus subtilis esterase: characterization and application for resolving dl-menthyl acetate. J. Mol. Catal. B: Enzym. 109, 1-8.

[15] Hu K, Li CX*, Pan J, Ni Y, Zhang XY, Xu JH* (2014). Performance of a new thermostable mannanase in breaking guar-based fracturing fluids at high temperatures with little premature degradation. Appl. Biochem. Biotechnol., 172, 1215-1226.

[16] Wang LJ, Li CX*, Ni Y, Zhang J, Liu X, Xu JH* (2011). Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor. Bioresour. Technol., 102, 7023–7028

[17] Ni Y, Li CX*, Zhang J, Shen ND, Bornscheuer UT, Xu JH* (2011). Efficient reduction of ethyl 2-oxo-4-phenylbutyrate at 620 g/L-1 by a bacterial reductase with broad substrate spectrum. Adv. Synth. Catal., 353, 1213–1217.

[18] Ni Y, Li CX*, Ma HM, Xu JH* (2011). Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity. Appl. Microbiol. Biotechnol., 89, 1111–1118.

[19] Ni Y, Li CX*, Wang LJ, Zhang J, Xu JH* (2011). Highly stereoselective reduction of prochiral ketones by a bacterial reductase coupled with cofactor regeneration. Org. Biomol. Chem., 9, 5463–5468.

[20] Qiu, LH, Li CX*, Sun J, Xu JH* (2011). Thermostable bacterial endoglucanases mined from Swiss-Prot database. Appl. Biochem. Biotechnol. 165, 1473–1484.

代表性发明专利：

[1] 李春秀、蒋莹、许建和、潘江、钱小龙。一株谷氨酰胺转氨酶高产诱变菌株的筛选及其应用。中国专利号：ZL 201710614341.1；授权日：2020.04.07

[2] 李春秀、郑明敏、潘江、许建和、钱小龙。7β-羟基甾醇脱氢酶突变体及其在熊脱氧胆酸合成中的应用。ZL 201710413074.1；授权日：2020.04.07     

[3] 李春秀、郑明敏、潘江、许建和、钱小龙。7β-羟基甾醇脱氢酶突变体及其在熊脱氧胆酸合成中的应用。中国专利号：ZL 201710413074.1；授权：2020.04.07

[4] 许建和、陈科材、李春秀、陈琦、潘江。沙雷氏菌脂肪酶突变体、重组表达转化子、酶制剂及应用。中国专利号：ZL 201610379653.4；授权日：2019.8.6

[5] 许建和、柳絮云、李春秀、罗晓晶、潘江。一种伯克氏菌株、其有机磷酯水解酶和基因及其在有机磷农药降解中的应用。中国专利号：ZL 201410169274.3；授权日2016.06.01   

[6] 许建和、胡可、李春秀、潘江。 甘露聚糖酶及其基因和应用。 中国专利号：ZL 201310283115.1；授权日：2015.02.25。

[7] 许建和、周冬杰、潘江、李春秀、郁惠蕾、郑高伟。一种产酯酶肠杆菌、其酯酶和基因及其在制备紫杉醇手性前体中的应用。ZL 201210339852.4；2014.06.11

[8] 许建和、张陈胜、潘江、李春秀、张志钧。一种腈水解酶及其基因和应用。中国专利号：ZL 201210044367.4；授权日：2014-10-01 06.11。

[9] 马宏敏、李春秀、潘江、郑高伟、郁惠蕾、许建和。一种羰基还原酶、基因和突变体及在不对称还原羰基化合物中的应用。中国专利号：ZL 201210136823.8；授权日：2014.06.11

[10] 许建和、倪燕、李春秀、张杰、潘江、王丽娟。一种还原酶及其基因、重组酶及制备方法和应用。中国专利号：ZL 201210050842.9；授权日：2014.02.12。

[11] 许建和、倪燕、潘江、李春秀、马宏敏。 生物催化不对称还原制备(R)-邻氯扁桃酸甲酯的方法。中国专利号：ZL 201110098532.X；授权日：2013.06.26。

[12] 许建和、沈乃东、钱小龙、张杰、郑高伟、李春秀、潘江。 一种羰基还原酶及其基因和在不对称还原羰基化合物中的应用。中国专利号：ZL 201110387573.0；授权日：2013.06.26。 

[13] 许建和、沈乃东、钱小龙、张杰、郑高伟、李春秀、潘江。一种羰基还原酶及其基因和在不对称还原羰基化合物中的应用。ZL 201110387573.0；2013.06.26

[14] 郁惠蕾、石乾乾、李春秀、许建和。一株青霉菌及其用于催化水解木质纤维素的应用。ZL 200910247272.0；2013.04.10

[15] 许建和、倪燕、李春秀、张杰、潘江、王丽娟。一种还原酶及基因、重组酶及制备方法和应用。ZL 201010527409.0；2013.04.24

联系方式
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